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For the resolution of L-carnitine from DL-carnitine, resting cells of Enterobacter sp. NH-104, which had a higher capacity of D-camitine decomposition, were harvested at maximal specific activity of D-carnitine decomposition of 47.05 unit/㎎ cell. The cells were frozen at -80℃ to assess functions as enzyme sources. Optimal concentration of cells and DL-carnitine were 17 g/l and 20 g/l, respectively, and reaction buffer was best at 75 mM of Tris·HCl. Optimal temperature and pH were 36℃ and 8.2, respectively. When the reaction at optimal conditions was carried out for 14 h, the optical purity was 98.21%, and the quantity and yield of remaining L-carnitine were 4.432 g/l and 44.32%, respectively.
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